Journal: Genetics and Molecular Research

Article Title: Characterization of yolk sac proteins of Bos indicus cattle embryos

doi: 10.4238/2012.november.14.1

Figure Lengend Snippet: Figure 2. Membranes after ECL showing the molecular weight (MW) and samples of the yolk sac of embryos 2 and 3 at an estimated gestational period from 30 to 40 days. Red arrows indicate the α-fetoprotein in the samples 2:03 with a molecular weight of 70 kDa.

Article Snippet: The secondary antibody was also diluted in TTBS and applied to the membrane for 1 h. Mouse anti-human α-fetoprotein monoclonal antibody (AFP; Serotec, MCA1863HT), mouse anti-human transferrin antibody (BD, 612124) and rabbit anti-human α-1-antitrypsin polyclonal antibody (A1AT; Abcan, AB922) were applied as primary antibodies, and anti-mouse- and anti-rabbitperoxidase conjugate as secondary antibody (GE, NIF825).

Techniques: Molecular Weight

Journal: Cell Death & Disease

Article Title: Androgen receptor-mediated apoptosis in bovine testicular induced pluripotent stem cells in response to phthalate esters

doi: 10.1038/cddis.2013.420

Figure Lengend Snippet: Pluripotency of bovine iPSCs. ( A ) In vitro differentiation of and marker expression by bovine iPSC-derived ectodermal, mesodermal, and endodermal precursor cells. Immunostaining with antibodies directed against the astrocyte-specific antigen GFAP (ectodermal differentiation), neuron-specific antigen Tuj1 (ectodermal differentiation), cardiomyocyte-specific antigen Nkx 2.5 (mesodermal differentiation), or α -fetoprotein (endodermal differentiation). ( B ) Teratoma formation 6–8 weeks after the transplantation of bovine iPSCs into SCID mice. Teratomas were sectioned and stained with hematoxylin and eosin. Immunohistochemical staining was performed using antibodies specific for S-100 (nerve bundles) and muscle-specific actin (mesenchymal cells and myofibroblasts) or PAS staining (secretory cells) ( × 400 magnification). In panel a, the red and yellow arrows indicate blood vessels and nerve bundles, respectively. In panel b, the red arrows indicate glands. S-100 staining indicates nerve bundles (panel c; red arrows), and muscle-specific actin staining indicates mesenchymal cells and myofibroblasts (panel d; red arrows). PAS staining indicates secretory cells (panel e; red arrows). The proliferation index of the whole teratoma was<3%

Article Snippet: The following antibodies were used: mouse anti-astrocyte-specific GFAP antibody (Sigma-Aldrich), mouse neuron-specific Tuj1 antibody (Sigma-Aldrich), mouse anti-cardiomyocyte-specific anti-human Nkx 2.5 antibody (CosmoBio, Tokyo, Japan), and mouse anti-endoderm-specific anti-human α -fetoprotein protein (CosmoBio).

Techniques: In Vitro, Marker, Expressing, Derivative Assay, Immunostaining, Transplantation Assay, Staining, Immunohistochemical staining

Journal: PLoS ONE

Article Title: Analysis of Mitochondrial Function and Localisation during Human Embryonic Stem Cell Differentiation In Vitro

doi: 10.1371/journal.pone.0052214

Figure Lengend Snippet: (a) Mitochondria in hESC are localised near the nucleus. (b) Mitochondria in AFP positive endoderm lineage cells. Mitochondria in AFP positive cells display a granular, dispersed localisation through the whole cell. (c and d) Mitochondria in MIXL1 positive cells (Mesendoderm) display a densely packed, perinuclear localisation based on MitoTracker far red (c) and LDS-751 (d) staining. AFP, alpha fetoprotein. Images (a-c) are 150 µm wide. Line profile in (d) represents 120 µm.

Article Snippet: Primary antibodies used were mouse IgG 1 anti-mitochondria (clone 113-1, 2 µg/mL), mouse IgG 1κ anti- Oct-4 (2 µg/mL), mouse IgG 3 anti-SSEA-4 (2 µg/mL), mouse IgG 1 anti-Tra-2-49 (2 µg/mL), mouse IgG 2a anti-TG30 (1 µg/mL), mouse IgG 2a anti-α-fetoprotein (AFP, 2 µg/mL), rabbit IgG anti-nestin (5 µg/mL) and mouse IgG 1 anti-MAP-2 (5 µg/mL), mouse IgG 1 anti-β3-tubulin, (all from Merck Millipore).

Techniques: Staining